The flow cytometer Diaries
The flow cytometer Diaries
Blog Article
At that time, the early indications were being dismissed as particles, although the retrospective analysis demonstrated shared properties with the problematic cells in lifestyle. Tracing the infection served the lab create further laboratory methods for screening and safety of assay-important mobile strains.
Whether or not you’re a beginner or a professional, explore our resources to advance your study and experience in flow cytometry.
Incorporate acoustic focusing technologies with superior-parameter Examination for flexible spectral unmixing and conventional workflows.
Explore new insights into your experimental information by visualizing populations making use of combined fluorescence and morphology information. Our program interface wizards permit downsampling, concatenation (merged data files) and dimensionality instruments which are crafted in to the application for an intuitive, streamlined knowledge Assessment encounter.
*Pricing is indicative only and it is matter to change. You should Speak to your Agilent agent with any thoughts. The information established forth herein isn't an offer or a determination, both of that happen to be subject matter to credit score acceptance, conditions and terms, and availability with Agilent. Agilent Financial Remedies is offered in chosen international locations.
Listed here, a highly skilled consumer has gated singlets confidently. Right after analyzing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate incorporates more than 4% aggregates.
Optimizing gating tactic. Even strong handbook singlet gating is error-susceptible and remains a subjective selection place in Just about all flow cytometry assays. Imaging can be utilized to confirm and change gates to include only one cells of curiosity.
Regular picture high-quality even at high throughput Acoustic concentrating as well as a superior-speed digicam combine to impression these T cells continuously at very low or superior flow prices. Effortlessly modify emphasis and digital camera configurations to satisfy experimental demands.
The Attune NxT cytometer is definitely the reputable workhorse instrument your lab can rely upon for constant and trustworthy efficiency. The Attune CytPix model contains a superior-speed brightfield camera and AI-pushed impression Assessment program, with the latest updates such as machine Understanding instruments and an intuitive user interface, enabling enhanced information precision, precision, and a lot quicker insights into sample biology.
People can check out occasion illustrations or photos in serious-time to substantiate sample top quality, enhance protocols, and report the morphology of each and every cell populace determined for Examination. Imaging seize also Rewards from acoustic focusing, which really helps to position the cells making sure that a pointy, centered picture is obtained.
Audio waves enable specific mobile alignment, leading to Outstanding details high quality and ~10X more rapidly sample flow costs.
We Earlier demonstrated the power of imaging Car or truck-T/Ramos cell interactions. Let’s evaluate just the population of finest curiosity, the double good occasions, To find out more. We can easily now use prolonged morphological parameters (right here, circularity and skewness of intensity) to even more study the features of those populations and refine gating on these gatherings, raising knowledge robustness.
Leverage the strength of Superior engineering and innovation to speed up your discoveries. Belief while in the Attune Flow Cytometer relatives to provide the trustworthiness, velocity, and precision that set your operate aside.
Versatile payment strategy from Agilent Economic Solutions allow you to get the significant instrumentation you will need for rapid challenge, or to develop your lab.
At the heart of every Attune Flow Cytometer lies our revolutionary acoustic concentrating technology, which utilizes seem waves to meticulously align cells within the check here flow mobile. This revolutionary solution surpasses regular hydrodynamic approaches, resulting in a lot quicker processing speeds, Outstanding information excellent, and elevated sample throughput.
Because the mobile line had previously been analyzed to the Attune CytPix Flow Cytometer, the researchers went back to the images and were able to doc the microbial infection no less than five times previously.
The Attune CytPix Flow Cytometer makes it possible for buyers to visualise samples throughout acquisition, verify the precision of gating approaches, and assistance success with responsible documentation for publication purposes.
Listed here, we display that by gating methods making use of image-dependent quantitative parameters can distinguish interacting cells from coincident events to extra correctly evaluate interacting cells.